FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_1_S13_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_1_S13_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22155780
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAAGC250.009768493130.26901138-139
GGATCCA351.7501607E-675.954612
AGACACT51550.067.1435851
CAAGCTG703.263267E-853.168233
GACACTG66150.052.0831642
GTGCATG950.007547988651.421978138-139
TCTTCCG276450.049.4852072
CTGCGCA350.0096343848.34275118-119
ACACTGC72350.047.5464363
GCACTGC455.5262516E-447.260653
CACTGCC73850.046.652694
TTCCGAT271700.045.888664
TGCCACA77150.044.588267
ACTGCCA77550.044.4953965
CTGCCAC77550.044.4953966
CTTCCGA292650.044.2568973
CGATCTG15550.042.739737
CCGATCT266900.041.4548846
GCCACAT83200.041.1542558
ATCTGGC656.84197E-540.8986368
Produced by FastQC (version 0.11.5)