FastQCFastQC Report
Mon 22 Jul 2019
Sealice_F2_S22_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F2_S22_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81699034
Sequences flagged as poor quality0
Sequence length150
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG827840.10132800346207275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT5050.0121.232341
GCCTGCT4350.0104.31391
CTTGGCT4700.0102.675741
TGTGCTC25250.0100.694152
TGCAGAC11400.099.154683
CTGGCTC20650.098.709122
TTGGCTC28300.094.6776352
TGGCTCT76500.093.173453
CTAGGCT2950.090.338031
GGCCATA4000.090.032831
CTGTGCT6100.089.737641
ATGGCTC17250.087.684162
GTGCTCT45600.087.470843
ACTGGCT5450.085.9028851
GGCCATC1100.085.1219561
TCTGGCT8350.084.533821
GCTGGCT3250.084.215331
TAGGCTC14500.083.451122
GGCCAAT2100.082.3157351
GGCCAAC2800.082.3157351
Produced by FastQC (version 0.11.5)