FastQCFastQC Report
Mon 22 Jul 2019
Sealice_F2_S22_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F2_S22_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81699034
Sequences flagged as poor quality0
Sequence length150
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT6500.0122.019671
GGCCATA2500.0115.364061
ACTGGCT5900.0114.875211
GCTGGCT4300.0114.022621
TCTGGCT7750.0110.712281
CTTGGCT6500.0109.8176961
CATGGCT3700.0107.179451
GGCCAAT1900.0106.256361
TTGGCTC31700.0105.310642
CTGGCTC26600.0105.172112
CGTGGCT1450.0104.4243551
TAGGCTC16400.0102.4383542
GGCCACT850.0101.791821
TGGCTCT91550.0100.971083
CTAGGCT3050.099.2887341
TGTGCTC10900.097.23922
GGCCAAA9600.096.136711
GGCCTAA3100.095.361421
GGCCAAC1750.094.763331
ATGGCTC19950.094.691042
Produced by FastQC (version 0.11.5)