FastQCFastQC Report
Mon 22 Jul 2019
Sealice_F2_S22_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F2_S22_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82177525
Sequences flagged as poor quality0
Sequence length150
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG928130.11294207266524514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT4450.0119.767731
GCCTGCT4100.0112.4253541
TTGGCTC26450.0104.561892
CTTGGCT5250.0104.261131
TGTGCTC26450.0100.477442
CTGGCTC18800.099.222422
GGCCAAT3200.099.030921
ACTGGCT4900.098.479741
TGCAGAC10400.095.540693
TCTGGCT6900.094.986181
TGGCTCT74200.092.282473
GGCTGCT1250.092.188791
CGTGCTC3500.090.542512
GGCCTAA3150.089.17071
GTGCTCT48550.089.1308063
CCGTGCT1700.088.9689561
TAGGCTC14100.087.3463752
CTAGGCT3300.087.299991
GCCCTGT1900.083.394461
CTGTGCT4150.083.303121
Produced by FastQC (version 0.11.5)