FastQCFastQC Report
Mon 22 Jul 2019
Sealice_F2_S22_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F2_S22_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82177525
Sequences flagged as poor quality0
Sequence length150
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT6750.0133.53311
GCTGGCT3100.0125.6072541
TCTGGCT7900.0121.39681
CTTGGCT5850.0117.098251
CTAGGCT3950.0116.833011
CATGGCT4150.0111.202491
ACTGGCT5650.0111.033351
TTGGCTC30050.0109.1816252
CGTGGCT1150.0106.5942461
TGTGCTC8800.0106.5229952
CTGGCTC27000.0106.0252762
CCGTGCT850.0101.799351
TGGCTCT91500.0100.086413
GGCCAAT3650.098.777911
ACAGGCT2650.097.957861
TAGGCTC15750.096.601652
GTGCTCT18700.096.25153
GGCCAAC2200.095.0512851
CCAGGCT3750.094.220951
GGCCATA2000.093.7402341
Produced by FastQC (version 0.11.5)