FastQCFastQC Report
Mon 22 Jul 2019
Sealice_F1_S20_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F1_S20_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112706276
Sequences flagged as poor quality0
Sequence length150
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1646960.14612850840710945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT43650.0127.884421
CCTGGCT16800.0125.191181
GCCAGCT10350.0119.698371
GGCTGCT17300.0118.2421651
CCGTGCT20600.0117.8318941
CTGTGCT39200.0116.67771
CAGTGCT19000.0115.623571
GCTTGCT10600.0115.516281
CGCTGCT13650.0113.450381
GCATGCT6800.0112.278631
TGCTGCT25250.0111.821371
GACTGCT11500.0111.48651
CTTGGCT16100.0110.054911
ACGTGCT8450.0109.959611
CATGGCT12400.0109.784311
CTAGGCT12500.0108.906041
CCAGGCT10300.0108.391361
TGCAGCT7000.0107.012731
GCTGGCT14550.0104.947721
ACTGGCT20850.0103.982581
Produced by FastQC (version 0.11.5)