FastQCFastQC Report
Mon 22 Jul 2019
Sealice_F1_S20_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F1_S20_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112706276
Sequences flagged as poor quality0
Sequence length150
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT22950.0130.69191
CATGGCT16950.0130.163671
GCTGGCT17650.0129.494871
GCTAGCT6450.0128.551211
CCGTGCT10450.0128.331911
CGCAGCT6050.0127.516521
CGCTGCT7250.0127.29461
CTAGGCT17000.0124.691391
GCTTGCT6550.0124.387051
CTTGGCT23100.0123.912911
GGCCTAA4250.0122.146671
ACTGGCT25150.0121.839741
GCCTGCT31800.0121.527821
GCGTGCT5750.0120.37641
GCATGCT4650.0119.392161
CAGTGCT12700.0117.5180741
CTGTGCT15900.0116.9931951
CCAGGCT14750.0115.849551
GCCTGAT16450.0115.273071
TGCCGCT2450.0114.7721561
Produced by FastQC (version 0.11.5)