FastQCFastQC Report
Mon 22 Jul 2019
Sealice_F1_S20_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F1_S20_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113474961
Sequences flagged as poor quality0
Sequence length150
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1848490.16289849176506876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGCT10900.0128.84741
CCTGGCT14250.0126.355071
GCCTGCT43900.0125.670051
GGCTGCT16150.0123.530681
GACTGCT11300.0122.374331
CGCTGCT14050.0122.0023351
CCGTGCT22100.0121.558161
CTGTGCT36800.0119.776361
CGCAGCT3050.0118.0694961
TGCAGCT5800.0117.9677051
CAGTGCT22600.0117.9127661
GTCAGCT7150.0117.854831
CATGGCT9700.0117.314831
ACTGGCT20100.0115.737481
GCTTGCT9400.0115.695541
GCGTGCT11750.0114.622871
GGCCATA6550.0113.2565841
GCATGCT6100.0112.166021
GCTGGCT13350.0111.135671
TGCTGCT26750.0110.65871
Produced by FastQC (version 0.11.5)