FastQCFastQC Report
Mon 22 Jul 2019
Sealice_F1_S20_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSealice_F1_S20_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113474961
Sequences flagged as poor quality0
Sequence length150
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT22800.0135.358221
GCTGGCT15650.0134.0771
CGCAGCT6950.0132.800841
CCGTGCT10750.0130.127461
ACTGGCT24700.0129.033051
GCTTGCT6550.0128.801281
GCTAGCT7700.0127.357291
CTAGGCT18700.0127.247121
CGCTGCT9600.0126.186741
CATGGCT18950.0125.5684051
GCCTGCT31500.0124.984951
CTGTGCT16200.0124.183761
CTTGGCT22200.0123.101091
CAGTGCT9750.0122.766291
GCGTGCT5550.0122.126681
CGTAGCT1950.0122.026741
ACAGGCT13050.0121.55921
ACGTGCT4400.0121.270381
GCCTCAG16650.0119.961311
TGCAGCT12150.0119.88111
Produced by FastQC (version 0.11.5)