FastQCFastQC Report
Mon 22 Jul 2019
CTRL_8C_26psu_2_S18_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_2_S18_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16972486
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG435490.2565858649110095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCC100.00723326144.04561
CATGGCT750.0144.045581
CTTGGCT1050.0130.326971
CCTGCGG3450.0123.169421
CCTGGCT1850.0120.686841
GCCTGCT2300.0118.994181
CTGTGCT2850.0111.1930851
GCTGGCT1450.0109.275961
TTGGCTC7500.0108.514342
CTGGCTC6600.0105.851682
CCCTGCT7550.0103.979931
TGCAGAC5750.0103.926893
TAGGCTC4100.0101.88592
TGGCTCT22250.0101.605523
ACTGCGG3150.098.3168261
GCCAGCT1100.098.2129061
ACTGGCT1100.098.2129061
GGCTGCT451.1439624E-896.030391
TCTGGCT2200.094.939141
ATGGCTC5100.093.205972
Produced by FastQC (version 0.11.5)