FastQCFastQC Report
Mon 22 Jul 2019
CTRL_8C_26psu_2_S18_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_2_S18_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16972486
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTAC100.0072094877144.204281
ACTGGCT2700.0141.533841
CTTGGCT2650.0141.483441
CCTGGCT3650.0132.351881
CATGGCT2300.0131.664781
CCTGCGG7900.0131.42671
GCTGCGA1450.0124.314031
GCTGGCT1200.0120.1702351
ATGGCTC8150.0115.009562
TCTGGCT3900.0114.623921
CTGCGGT13050.0114.368912
CCCTGGG2350.0113.522531
ACAGGCT1050.0109.8699341
TTGGCTC15350.0108.9752352
CTTGCGG2950.0107.542181
CTGGCTC13500.0107.352082
TAGGCTC5900.0106.3201142
GGCCTTT750.0105.749811
CTGCGGA9900.0105.604162
TGGCTCT42150.0105.3853453
Produced by FastQC (version 0.11.5)