FastQCFastQC Report
Mon 22 Jul 2019
CTRL_8C_26psu_2_S18_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_2_S18_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16982389
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG477590.2812266283618871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGCT1050.0144.052021
CCTGGCT1750.0135.820471
GCCTGCT1550.0134.758331
CTTGGCT1550.0130.11151
GCTGGCT1100.0124.408561
CAGTGCT550.0117.860741
ACTGCGG2750.0117.860731
TGCAGAC6050.0117.81913
CTGTGCT2400.0114.041191
CCTGCGG3900.0112.656071
TGTGCTC8700.0109.2805252
TGGCAAC203.9693617E-4108.039011
CCAGGCT1150.0106.4732361
CTGGCTC6750.0105.637842
TTGGCTC8150.0103.398992
CCTTGCT3350.0103.201451
TGCTGCT1050.0102.89431
CCCTGCT7400.0101.225741
GGCCTTT502.055458E-10100.836411
ACTGGCT1500.0100.836411
Produced by FastQC (version 0.11.5)