FastQCFastQC Report
Mon 22 Jul 2019
CTRL_8C_26psu_2_S18_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_2_S18_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16982389
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATGG500.0144.215591
CCTGGCT3250.0141.996891
CATGGCT2150.0137.507891
TCTGGCT4050.0137.093841
CCTGCGG8400.0134.772921
ACTGGCT2550.0127.249061
GCTGGCT1650.0126.7349241
GGCCTAT403.45608E-11126.1886441
CTTGGCT3050.0125.302071
CTAGGCT1700.0123.007421
GCCCTGT600.0120.1796651
GCCCTTG550.0117.9945761
ACTGCGG5450.0115.1078641
ACAGGCT1350.0112.167691
GCTGCGA1550.0111.650791
GCCTAGG650.0110.9350741
TCCTGGG1500.0110.565291
CTGTCGG3200.0110.415061
CCAGCGG5300.0110.202491
CTGGCTC14000.0109.706862
Produced by FastQC (version 0.11.5)