FastQCFastQC Report
Mon 22 Jul 2019
CTRL_8C_26psu_1_S17_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_1_S17_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13627098
Sequences flagged as poor quality0
Sequence length150
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG404290.2966809220862725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCATA306.82121E-10144.04971
GCCCTTG100.0072326297144.049681
CCTGCGG1500.0129.644711
GCCTGCT1500.0124.843061
CTTGGCT1100.0124.406541
TGCTGCT351.9936124E-9123.471161
CCTGGCT1050.0123.471151
CATGGCT501.8189894E-12115.239751
GCTGGCT950.0113.7234341
GCCCTGT458.913048E-11112.038641
TCTGGCT1550.0111.522341
GCCTTGG203.9696004E-4108.037261
CTGGCTC4350.0107.623322
CTGTGCT1150.0106.4715041
TGTGCTC4950.0104.7634052
CTGGGCT553.6379788E-12104.7634051
TTGGCTC4600.0103.339992
TGGCTCT15000.0102.716983
GGCCAAT650.099.726711
ATGGCTC3800.096.664922
Produced by FastQC (version 0.11.5)