FastQCFastQC Report
Mon 22 Jul 2019
CTRL_8C_26psu_1_S17_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_8C_26psu_1_S17_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13781290
Sequences flagged as poor quality0
Sequence length150
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG440640.31973784747291434No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCACT202.2026288E-6144.047941
CTTGGCT650.0121.886721
CTGTGCT1100.0117.857411
ACTGCGG1600.0112.537451
CCTGGCT1050.0109.75081
CTGCGGT3550.0109.557592
ACTGGCT1400.0108.035961
CCTGCGG1600.0108.035951
GACCTTG203.9697927E-4108.035951
TGTGCTC5050.0106.96632
GCCTGCT1400.0102.891391
ATGGCTC2950.0102.54262
TGCCGAA2250.0102.434081
CTGGCTC4600.0100.207262
TCTGCGG2800.097.746821
TAGGCTC3400.097.44422
GGCACAT301.6533966E-596.031961
TGCAGAC2550.096.000253
TGGCTCT14700.095.510463
TTGGCTC4900.094.072122
Produced by FastQC (version 0.11.5)