FastQCFastQC Report
Mon 22 Jul 2019
CTRL_16C_26psu_2_S21_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_2_S21_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13408232
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCCT100.0072318884144.054611
CAGGGCT202.2021195E-6144.054611
GGCTGCT4000.0136.85191
CCTGGCT2300.0131.528121
CCTGCGG10300.0130.768021
CTTGGCT2600.0127.432931
GCCACGG1250.0126.768051
GCATGCT1200.0126.047791
CAGTGCT3850.0125.346221
GCCTGCT9600.0123.046651
GCTGCGG10900.0122.248181
GCTGGCT1900.0121.309141
CATGGCT1550.0120.820011
CCTGCGA5900.0117.1969761
TGCTGCT4750.0116.760061
CTGTGCT8850.0115.5692441
ACTGCGG12700.0115.130271
GACTGCT1950.0114.504951
CTGCGGT22650.0114.162482
GCCCTGT700.0113.1857761
Produced by FastQC (version 0.11.5)