FastQCFastQC Report
Mon 22 Jul 2019
CTRL_16C_26psu_2_S21_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_2_S21_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13408232
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTA253.8400685E-8144.191151
TGCAGCC100.0072114365144.191151
ACTGGCT2600.0135.872421
GGCCTAT700.0133.891771
CCTGCGG15300.0133.353241
CCTGGCT3200.0132.926211
GCTTGCT550.0131.082861
GCTGGCT2700.0130.84011
GCCTCGG8050.0128.965991
ACTGCGG15000.0128.810751
GCCAGCT2300.0128.518191
ACAGGCT2050.0126.606861
GGCAGCT800.0126.167251
GGCCGCT403.45608E-11126.167251
GCTGCGG13000.0124.780791
GGCTCGG4800.0124.665261
GCCCTGG351.9790605E-9123.592411
GCATGCT351.9790605E-9123.592411
TGCTCGG5650.0122.4986651
GCTGCGA7350.0121.630621
Produced by FastQC (version 0.11.5)