FastQCFastQC Report
Mon 22 Jul 2019
CTRL_16C_26psu_2_S21_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_2_S21_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13433110
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144150.10730947636102138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTT400.0144.051221
CCTGCGG9850.0129.426731
GCCTGCT7600.0128.887941
CAGTGCT3650.0128.264791
GCCAGCT1750.0127.588231
GGCTGCT2750.0125.717431
ACTGCGG12100.0125.003131
CCTGGCT2200.0124.4078751
GACTGCT2250.0121.643261
CTGTGCT7200.0121.043041
ACAGGCT1650.0117.860091
TGCAGCT1100.0117.860091
GCATGCT1050.0116.6128851
GCTGCGG11250.0115.881211
GCCATGT256.6823814E-6115.240981
GCCACGG1700.0114.393611
GCTGGCT1900.0113.7246551
CTTGGCT2350.0113.402021
GCTTGCT2100.0113.18311
TGCTGCT4500.0112.039841
Produced by FastQC (version 0.11.5)