FastQCFastQC Report
Mon 22 Jul 2019
CTRL_16C_26psu_2_S21_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_2_S21_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13433110
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCAA100.0072080814144.21361
GGCCCAT151.2572868E-4144.21361
GCCTGTC100.0072080814144.21361
GCAGCCG100.0072080814144.21361
GGCCTGA100.0072080814144.21361
GTTGCCC100.0072080814144.21361
CATGGCT2150.0134.152161
CCTGCGG14550.0133.310841
CCTGGCT2600.0133.120241
CTGTGCT2150.0130.798371
TGCTCGG5500.0129.792221
ACTGGCT2500.0129.792221
GCCTCGG8450.0129.70691
ACTGCGA9200.0126.970661
GCTGCGG15650.0126.705221
CTAGGCT2050.0126.626551
CCTGCGA7750.0126.53581
GGCCAAT403.45608E-11126.186891
GGCCTAA1150.0125.4031141
GCCAGCT2650.0125.1665041
Produced by FastQC (version 0.11.5)