FastQCFastQC Report
Mon 22 Jul 2019
CTRL_16C_26psu_1_S19_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_1_S19_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18490322
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240420.1300247772861933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT1550.0134.754141
CATGGCT1050.0130.328721
ACTGGCT1850.0120.688471
GCCTGCT1800.0120.039611
CCTGCGG3700.0116.7952961
CTTGGCT2350.0116.463961
GCTGGCT950.0113.721731
GGCCATA1150.0112.732851
CTGCGGT7850.0106.430022
GGCTGCT553.6379788E-12104.761841
CTGGCTC8400.0104.605942
ACAGGCT1250.0103.7142261
TGTGCTC10150.0102.18152
TCTGGCT3350.0101.048271
ATGGCTC6000.0100.8332752
CTGTGCT2600.099.725211
TTGGCTC9650.099.2659152
ACTGCGG3850.099.149611
TGGCTCT28400.096.842193
GGCCATT750.096.0316851
Produced by FastQC (version 0.11.5)