FastQCFastQC Report
Mon 22 Jul 2019
CTRL_16C_26psu_1_S19_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_1_S19_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18490322
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGCT2650.0141.483431
CCTGCGG7950.0134.227871
CCTGGCT2200.0131.094791
CTAGGCT2050.0130.135561
CATGCGG1950.0129.414081
CATGGCT1500.0124.977021
ACTGCGG8050.0121.812311
GCTGGCT1250.0121.1315841
TCTGGCT3300.0120.170211
CTTGGCT2250.0118.567951
CTGTGCT1050.0116.736781
GCCCTTG501.8189894E-12115.363411
GTGCCAA256.6472676E-6115.363411
GCAGGCT750.0115.36341
TTGGCTC13450.0113.647982
CCAGGCT1650.0113.615481
CTGCGGA9300.0113.19262
CTGCGGT15100.0112.689432
TAGGCTC7050.0110.454342
GCCATGG405.007678E-9108.1532061
Produced by FastQC (version 0.11.5)