FastQCFastQC Report
Mon 22 Jul 2019
CTRL_16C_26psu_1_S19_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_1_S19_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18576202
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG261030.1405184978070329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCGG3450.0125.259981
CCTGGCT1550.0120.815271
CATGGCT900.0120.040811
CTTGGCT1500.0115.239181
CCAGGCT900.0112.038091
GGCCATA1300.0110.80691
GGCCCAT203.969701E-4108.0367361
ACTGCGG4200.0108.036731
CTGTGCT2400.0108.036731
TAGGCTC4450.0106.822842
TGCTGCT1300.0105.2665561
GCCTGCT2200.0104.762891
GGCTGCT553.6379788E-12104.762891
GACTGCT700.0102.892131
TTGGCTC9550.0102.56892
CTGCGGT9500.0102.3505862
GCTGCGG3800.0102.350581
ACTGGCT1900.0102.350581
CAGGGCT301.6533528E-596.0326461
TGTGCTC11200.095.175222
Produced by FastQC (version 0.11.5)