FastQCFastQC Report
Mon 22 Jul 2019
CTRL_16C_26psu_1_S19_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCTRL_16C_26psu_1_S19_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18576202
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCT400.0144.210051
CCTGGGC100.007208632144.210052
GGCTCAT100.0072410395143.993844
CCTGGCT2800.0139.05971
ACTGGCT2450.0138.323931
CCTGCGG7400.0136.414921
CATGGCT1600.0135.196931
GCTGGCT1250.0126.904851
CTTGGCT2000.0126.18381
GCCCTGT750.0124.982051
GCTGCGA1800.0124.180881
TCTGGCT2450.0123.608611
CTGGGCT850.0118.7612151
GGCCCTA800.0117.170671
ACTGCGG6850.0114.7364651
CTAGGCT1900.0113.850041
ACAGGCT900.0112.1633761
TTGGCTC12600.0110.4465942
CCAGGCT2100.0109.874331
CTGGCTC10800.0109.492822
Produced by FastQC (version 0.11.5)