FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_4_S8_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_4_S8_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14607942
Sequences flagged as poor quality0
Sequence length150
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGCT151.2629793E-4144.05021
CTAGGCT700.0123.4715961
TGCTGCT600.0120.041831
GCCCTGT900.0120.041831
CCTGCGG2700.0120.041831
CTTGGCT1150.0118.9979861
GCCATTG256.682638E-6115.2401662
GGCTGCT256.682638E-6115.2401661
GGCCATT458.913048E-11112.039051
GCCTGCT900.0112.039051
GGCAGCT203.9695494E-4108.037651
ATGCACG405.0458766E-9108.037651
GGCCCTA553.6379788E-12104.763791
CCTGGCT1100.0104.763791
GCTGGCT850.0101.6824951
GGCCATA850.0101.6824951
CTGTGCT1800.096.033471
TGCCGAA1350.096.033471
TGTGCTC8100.096.033462
GCAGGCT301.6532713E-596.033461
Produced by FastQC (version 0.11.5)