FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_4_S8_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_4_S8_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14607942
Sequences flagged as poor quality0
Sequence length150
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGCT900.0144.198971
CCTGCGG4400.0139.28311
CCTGGCT2050.0133.647831
GGCCATA1150.0125.390421
CATGGCT1700.0122.993241
ACTGGCT1550.0120.941081
TCTGGCT2050.0119.5796361
CCAGGCT1000.0115.359171
CTGGCTC7350.0113.789672
CTAGGCT1600.0112.655451
GGCCCTA458.913048E-11112.1547551
GGCCAAC650.0110.922291
CTGCGGA4200.0109.865882
ATGGCTC5800.0109.392332
GGTCCCT203.9532833E-4108.149231
TGGCTCT27150.0106.065763
CAGTGCT553.6379788E-12104.871981
TGTGCTC3100.0104.6605452
TTGGCTC10050.0104.024132
CTTGGCT1850.0101.329011
Produced by FastQC (version 0.11.5)