FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_4_S8_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_4_S8_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14686084
Sequences flagged as poor quality0
Sequence length150
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGCT700.0144.048741
GCTTGCT151.2630306E-4144.048741
TGGCCAT151.2630306E-4144.048741
GCCTGCC100.007232779144.048741
CTTGGCT800.0135.045681
GCCTGCT750.0134.445481
CTGTGCT1050.0130.329821
CCTGGCT1200.0120.040611
CCTGCGG2800.0115.753451
CATGGCT950.0113.722691
TGCTGCT458.913048E-11112.0378951
GGCTGCT650.0110.806721
CTGGCTC5050.0108.3931052
GGCCATT203.9697118E-4108.0365451
TCTGGCT1800.0104.035191
ACTGCGG2150.0103.849081
GCTGCGA1050.0102.891961
ACTGGCT1200.0102.0345151
TGCAGAC3050.0101.508663
CTGCGGT5950.098.049982
Produced by FastQC (version 0.11.5)