FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_4_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_4_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14686084
Sequences flagged as poor quality0
Sequence length150
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACC100.0072070598144.220471
CCTGGCT1800.0144.220471
CTGTGCT700.0144.220471
CATGGCT1000.0137.009451
GGCCCTA750.0134.605771
GCTGGCT1000.0129.798421
CCTGCGG4100.0126.6326141
GCCTGCT1150.0125.40911
CTTGGCT1900.0121.4488141
GCCTTGG550.0117.998571
ACTGCGG2850.0116.388451
TCTGGCT2850.0116.388451
ACTGGCT1500.0115.376381
GGCAATC256.6435114E-6115.376371
CTGGCTC8000.0111.770872
CTTGCGG1500.0110.569031
TGTGCTC4050.0108.610472
GGCCTAT600.0108.165361
GCCATGG203.9509404E-4108.165351
TGCTCAG203.9509404E-4108.165351
Produced by FastQC (version 0.11.5)