FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_3_S7_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_3_S7_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14120008
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208370.1475707379202618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCATT202.2021723E-6144.053961
CCTGGCT1300.0127.432361
GGCCACT256.681763E-6115.243171
CCTGCGG1450.0114.249691
CATGGCT405.0440576E-9108.040471
GGCCATA800.0108.040471
ACTGGCT750.0105.639571
ACTGCGG1350.0101.37131
TGTGCTC5000.0100.837772
GCCTGCT800.099.0370941
TGCAGAC2200.098.178963
TTGGCTC4250.096.600892
TGGCTCT14000.091.540193
ATGGCTC3250.090.86482
CTGGCTC4600.087.685022
GCCATGT259.6366624E-486.432381
CTTGGCT750.086.432381
CTGCCGA259.6366624E-486.432381
TGCAGCT259.6366624E-486.432381
GGCCTTC259.6366624E-486.432381
Produced by FastQC (version 0.11.5)