FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_3_S7_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_3_S7_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14120008
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGCT1000.0144.207921
GTGGCCT100.0072089336144.207922
GCCATGT100.0072089336144.207921
GGCCCTA253.837522E-8144.207921
GGCCACT202.1904962E-6144.207921
GGCCTTA151.2574851E-4144.207921
CTTGGCT1450.0139.235231
CCTGGCT1950.0133.114991
TCTGGCT1750.0127.727011
CCTGCGG3250.0124.240661
ACTGCGG2800.0115.8813551
ACTGGCT2150.0114.0248641
GGCCACA458.913048E-11112.161711
ATGGCTC5100.0111.690442
CTGCGGT7400.0109.130312
GGCCTTT600.0108.155941
TGGCCTT203.9764485E-4107.9904863
TTGGCTC8900.0106.9407042
CATGGCT1500.0105.752471
GGCCAAA2200.0104.878471
Produced by FastQC (version 0.11.5)