FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_3_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_3_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14166205
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG230570.16276059819831776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGCT1000.0144.053121
CCTGGCT1500.0129.647811
CCTGCGG2200.0121.1355741
ACTGGCT850.0118.631991
CCAGGCT501.8189894E-12115.242491
GCCAGCT256.6819575E-6115.242491
CTAGGCT1000.0115.242491
CTGTGCT1200.0114.042051
CTTGCGG800.0108.039851
CTGCGGT3600.0106.03912
GCCTGCT1450.0104.314341
CATGGCT650.099.729081
TGCAGAC2400.099.003663
TTGGCTC5250.098.779292
ACTGCGG1700.097.44771
CTGGCTC5100.097.44772
CTGCGAT2000.097.235852
ATGGCTC4400.096.581062
TGGCTCT16700.096.5784153
TGTGCTC4700.095.013762
Produced by FastQC (version 0.11.5)