FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_3_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_3_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14166205
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCAA100.007209293144.20551
CCTGGCT2350.0135.00091
CCTGCGG3100.0134.901921
CTAGGCT700.0133.905121
TCTGGCT2750.0128.4741
CTTGGCT1650.0126.726041
ACTGGCT1400.0118.454531
GCTGGCT750.0115.36441
GGCCACT256.646931E-6115.36441
GCCCTGT750.0115.36441
CATGGCT1200.0114.162681
CTGGCTC8150.0112.356432
CTGCGGT6400.0111.533952
TAGGCTC3900.0109.078522
TGGCTCT27750.0107.671013
TTGGCTC10150.0107.266162
ATGGCTC5600.0106.8665852
CTTGCGG1900.0106.256691
GGCCCTA700.0103.003941
CAGGCTC3250.0102.053132
Produced by FastQC (version 0.11.5)