FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_2_S6_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_2_S6_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12868767
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG173370.13472153159661682No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCAT151.2630304E-4144.048651
TGGCTAC100.0072327834144.048631
CCTGGCT1550.0134.755171
CTTGGCT1050.0130.329711
GCCTGCT1750.0127.585941
CCTGCGG1450.0124.1798551
CATGGCT650.0121.887311
GCCCTGT301.14661816E-7120.0405351
ACTGGCT850.0110.154841
GCATGCT203.969711E-4108.0364761
CTGGCTC5450.0104.402222
GGCCTTT700.0102.8918841
TGCTGCT502.055458E-10100.834041
CTGTGCT2000.0100.834041
ACAGGCT650.099.725981
TGCAGAC2250.099.197653
GCTGGCT950.098.559591
TGTGCTC5550.096.0324252
GGCCATA607.2759576E-1296.0324251
GGCCTAA900.096.032421
Produced by FastQC (version 0.11.5)