FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_2_S6_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_2_S6_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12868767
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGCT900.0144.2041
CACCTGG100.007209511144.2041
GGCCATT253.8380676E-8144.2041
GGCCACT151.2576187E-4144.2041
CCTGGCT2050.0137.169651
GCTGGCT1400.0133.903721
CTTGGCT2050.0133.652481
CCTGCGG3750.0132.667661
GCCCTGT450.0128.181321
ACTGGCT1950.0122.018771
TCTGGCT3200.0121.672131
CCAGCGG1900.0117.64011
GGCCCTA501.8189894E-12115.36321
CTGGCTC9850.0110.5322
TTGGCTC9600.0109.655122
CCTGCGA1250.0109.595031
GCCTTGG203.952725E-4108.1531
ACAGGCT1000.0108.1531
CAGTGCT203.952725E-4108.1531
CTGCGGT6200.0108.152992
Produced by FastQC (version 0.11.5)