FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_2_S6_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_2_S6_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12939154
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190490.14721982596389221No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCAAT351.9936124E-9123.466071
GCCCTGT351.9936124E-9123.466071
CTTGGCT1000.0122.4371951
CCTGGCT950.0121.31
CTGGGCT256.684095E-6115.235011
CTTGCGG900.0112.0340351
CATGGCT650.0110.8028951
CTGTGCT1450.0109.2745741
TCTGGCT1650.0109.124061
GCCCCTG203.970246E-4108.0328141
CCTGCGG2300.0106.4671251
ACTGGCT950.0106.1375051
ACAGGCT750.0105.632091
TGCTGCT553.6379788E-12104.75911
ATGGCTC4300.0103.84512
GGCCATA700.0102.88841
TTGGCTC5700.099.819412
CTGGCTC4500.099.229762
TGGCTCT17400.096.828193
GGCTGCT301.6536313E-596.0291751
Produced by FastQC (version 0.11.5)