FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_2_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_2_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12939154
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTGG202.1900541E-6144.213671
GGCACCT100.0072080656144.213671
GGCCACT100.0072080656144.213671
GGGCACT100.0072080656144.213671
CTGTGCT400.0144.213671
CTAGGCT1200.0138.204771
ACTGGCT1200.0138.204771
CATGGCT1150.0137.943531
CCTGCGG3000.0132.195881
CTTGGCT1750.0131.85251
CCTGGCT1800.0128.189931
GCTGGCT1100.0124.5481641
CCAGGCT1000.0122.581631
TCTGGCT1900.0121.4430851
CAGGGCT301.1388147E-7120.1780551
CAGTGCT301.1388147E-7120.1780551
GGCCTAT600.0120.1780551
ACAGGCT850.0118.7642061
GGCCATA650.0110.9335861
ATGGCTC5450.0109.814082
Produced by FastQC (version 0.11.5)