FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_1_S5_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_1_S5_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13980098
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233780.1672234343421627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT1900.0132.675351
CCTGCGG3750.0132.523731
CCTGGCT1950.0129.27341
CATGGCT800.0126.041591
CTTGGCT1450.0124.1788941
ACTGCGG2250.0115.238021
GCCATGG256.6832436E-6115.238021
ACTGGCT1250.0115.238021
GCTGGCT650.0110.8057861
CTAGGCT1050.0109.75051
CTGCGGT6100.0108.626012
TGCTGCT1000.0108.035651
TGCAGAC3200.0107.995853
CTGGCTC5700.0107.403862
GCTGCGA1350.0106.7018661
CTGTGCT2050.0105.4006351
CCTGCGA1300.0105.2654951
CAGTGCT1150.0100.206981
TTGGCTC6550.0100.0635452
GGCCTTT800.099.032681
Produced by FastQC (version 0.11.5)