FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_1_S5_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_1_S5_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13980098
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGCT1350.0144.209171
GGCAGCT100.007208746144.209171
GGCACTT100.007208746144.209171
GGCCCAT151.2574418E-4144.209151
GGCCTAA600.0144.209151
CCTGCGG6050.0134.674681
GCTGGCT1300.0133.116151
CTAGGCT1300.0133.116151
CCAGGCT1250.0132.672421
CCTGGCT2450.0132.436981
GCCTCGG1600.0130.689541
TCTGGCT2600.0127.569641
CTTGGCT2050.0126.622671
ACTGGCT2000.0126.1830141
CATGCGG1950.0118.325461
CTTGCGG2400.0117.169941
TCAGGCT1000.0115.3673251
GCCCTTG256.646089E-6115.3673251
GCTAGCT501.8189894E-12115.3673251
ACTGCGG5700.0113.849341
Produced by FastQC (version 0.11.5)