FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_1_S5_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_1_S5_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14000021
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262630.18759257575399352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCT400.0144.0511
CCTGGCT1500.0139.24931
GCCTGCT1800.0136.048161
CCTGCGG4000.0135.04781
CATGGCT750.0134.44761
GCTGGCT750.0134.44761
CTTGGCT1050.0123.4722751
CAGTGCT1000.0122.4433441
ACTGGCT1000.0122.4433441
GCCCTGT900.0120.0424961
CTGTGCT2500.0115.24081
CTAGGCT1100.0111.3121261
CTGGCTC5700.0111.197262
CTTTGCT1750.0111.125051
CTGCGGT6500.0109.700372
GGCCCAT203.9694598E-4108.0382461
GGCCAGT203.9694598E-4108.0382461
ACTGCGG2950.0107.427861
TGTGCTC8000.0106.237612
GCTGCGA1300.0105.2680361
Produced by FastQC (version 0.11.5)