FastQCFastQC Report
Mon 22 Jul 2019
8C_32psu_1_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_1_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14000021
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCACC100.007208223144.212661
GGCCTTG100.007208223144.212661
GTGCCTA100.007208223144.212661
CACGTGC100.0072418833143.98818
CCTGGCT2650.0141.491671
CCTGCGG7000.0138.032121
ACTGGCT2300.0137.942541
CTTGGCT2300.0134.807481
CATGGCT1350.0133.530241
GGCCATA650.0133.119381
GCTGCGA1900.0132.827441
GGCCTAA500.0129.79141
GCTGGCT1100.0124.54731
GGCCAAT351.9772415E-9123.610851
GCCTTGG650.0122.02611
CCTGCGA2450.0120.667731
TCTGGCT2450.0120.667731
CATGCGG1500.0120.1772161
GGCACAT301.1388511E-7120.177211
CTGCGAT4700.0119.6658252
Produced by FastQC (version 0.11.5)