FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_4_S12_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_4_S12_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12486748
Sequences flagged as poor quality0
Sequence length150
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1045140.8369993532343248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT1100.0137.506591
GCCTGCT1300.0121.89231
CATGGCT553.6379788E-12104.766931
TGCAGAC2750.0104.724143
CTTGGCT1250.0103.719261
GACTGCT352.8734758E-7102.8960951
CTGGCTC4300.0102.1782152
TTGGCTC3950.0102.11462
TGGCTCT12150.098.960013
ATGGCTC2450.091.1365362
TGTGCTC3100.090.6149442
ACTGGCT1300.088.648951
CCTGCGG900.088.033321
GTCAGCT502.3763278E-886.432711
GTGCCTA259.636486E-486.432711
GGCCATA608.749339E-1084.031811
TCTGGCT1550.078.997641
TCCTGCT3950.076.585951
GGCTCTT24600.075.217264
TGCCGAA1650.074.209911
Produced by FastQC (version 0.11.5)