FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_4_S12_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_4_S12_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12486748
Sequences flagged as poor quality0
Sequence length150
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGCT1950.0136.793671
CCTGGCT2200.0134.356921
CATGGCT1450.0134.243931
TCTGGCT1950.0129.399411
ACTGGCT1700.0127.224631
GGGCCAT301.1400152E-7120.156591
CTAGGCT800.0117.152681
CCTGCGG1600.0112.646811
GGCCTAT458.913048E-11112.146161
CCTGAGT458.913048E-11112.146162
TTGGCTC9850.0111.983512
CTGGGCT650.0110.913791
CTGTGCT650.0110.913791
ATGGCTC6800.0109.201142
GCTGCGA600.0108.140941
CCAGGCT950.0106.243741
TGGCTCT28050.0105.7457353
GGCCTAA750.0105.737811
GCTGGCT900.0104.135711
ACTGCGG1600.0103.635061
Produced by FastQC (version 0.11.5)