FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_4_S12_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_4_S12_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12592704
Sequences flagged as poor quality0
Sequence length150
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1107020.8790963402300253No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGTG151.2632101E-4144.043471
CCTGGCT950.0128.881011
GCCTGCT1100.0111.306321
GGCCAAT352.8747854E-7102.888191
CTAGGCT352.8747854E-7102.888191
CCTGCGG850.0101.677741
TTGGCTC4350.0100.99562
CTTGGCT1200.096.0289841
TGTGCTC4050.094.25032
CCTTGCT1150.093.941391
CTGTGCT1500.091.227531
CATGGCT406.367609E-790.027171
TGGCTCT13100.089.589213
TGCAGAC2350.088.852483
CTGGCTC4250.088.120372
CCCTGCT3100.085.961421
GTGCTCT7650.085.6484153
TAGGCTC1600.085.525472
CAGGCTC1350.085.358752
TCTGGCT1450.084.439281
Produced by FastQC (version 0.11.5)