FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_4_S12_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_4_S12_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12592704
Sequences flagged as poor quality0
Sequence length150
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATGG151.2570123E-4144.221451
CCTGGCT1450.0144.221441
CTTGGCT1750.0135.980211
CCTGCGG1650.0117.999361
CCAGGCT1350.0117.513761
ACTGGCT1850.0116.93631
GCTGGCT650.0110.939571
TTGGCTC7950.0110.660472
CATGGCT1450.0109.409361
CTGCGGT2450.0108.90192
GGTCCAA203.9508214E-4108.1660841
CTTGCGG405.00404E-9108.1660841
GCAGGCT405.00404E-9108.1660841
TCTGGCT2150.0107.327581
TGCAGAC8050.0106.429153
TAGGCTC3950.0105.88412
CTAGGCT1100.0104.888321
CTGGCTC7050.0103.307552
TGGCTCT23400.0102.456113
ATGGCTC5250.0101.641782
Produced by FastQC (version 0.11.5)