FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_3_S11_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_3_S11_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17175482
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG264680.15410338993688796No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTC100.0072317724144.05551
CCTGCGG3850.0129.08871
CTTGGCT1700.0122.8708651
ACTGGCT1300.0116.352511
CCTGGCT1950.0114.5056461
GCCTGCT2100.0113.186461
TGCTGCT900.0112.043161
CATGGCT1050.0109.756571
CTGGCTC6250.0108.3297352
CTGTGCT2000.0108.041621
TGTGCTC8250.0106.513772
GGCCATA1300.0105.271321
CTGCGGT7300.0103.6015552
GTCAGCT352.873403E-7102.896791
CTAGGCT1050.0102.896781
TGGCTCT23200.0100.238263
CAGGCTC2450.099.956892
GGCTGCT650.099.730721
ATGGCTC4850.099.502252
GCTGCGG3700.099.2814941
Produced by FastQC (version 0.11.5)