FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_3_S11_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_3_S11_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17175482
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGCT253.837158E-8144.210661
CCTGCGG8650.0140.876311
CCTGGCT3200.0139.704071
CATGGCT2750.0131.100591
TCTGGCT3850.0131.100591
CTTGGCT2400.0129.188721
GCAGGCT950.0129.030581
ACTGGCT1750.0127.729441
GGCCATA1500.0120.175551
CTAGGCT2000.0118.973791
TTGGCTC13250.0118.089482
CATGCGG1850.0116.927561
GCTGGCT1300.0116.477831
ACTGCGG5800.0115.617161
TGCCTGT256.6457906E-6115.368531
CTTGCGG2900.0114.373961
CCTGCGA2650.0114.280141
GGCCAAT700.0113.3083651
CCAGCGG5900.0112.4354251
CTGCGAT5000.0111.04222
Produced by FastQC (version 0.11.5)