FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_3_S11_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_3_S11_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17221011
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG292720.16997840602970404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT1600.0135.041581
CCTGGCT2000.0129.639911
CATGGCT1200.0126.03881
ACTGGCT1500.0124.838441
GCTGGCT800.0117.036031
CTTGGCT2000.0115.235481
CCTGCGG3450.0114.8179551
TTGGCTC9700.0109.1472
CTGCGGC405.0476956E-9108.0332642
CTGGCTC7350.0107.788292
ATGGCTC5350.0106.35052
TCTGGCT2450.0105.828511
CTGCGGT7000.0104.9465942
CTGTGCT1800.0104.032031
TGGCTCT26900.0103.316293
CAGTGCT1050.0102.8888241
GCAGGCT352.8747309E-7102.888821
GGCCAAT502.055458E-10100.831041
TGCAGAC5600.0100.286013
GACTGCT650.099.723011
Produced by FastQC (version 0.11.5)