FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_3_S11_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_3_S11_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17221011
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAC151.2576254E-4144.204011
TGTCAGC100.0072095315144.2042
GGGCTCA100.0072095315144.2041
CCTGGAC100.0072095315144.2042
CCTGGCT2600.0138.65771
ACTGGCT2300.0137.934271
CCTGCGG9300.0137.22641
CTTGGCT2300.0134.79941
CATGGCT1750.0131.843671
GCTGGCT1100.0131.094561
GGCCTAT500.0129.78361
GGCCCTA450.0128.181341
CTAGGCT1600.0126.17851
GCCCTTG351.9790605E-9123.603441
CCTGCGA2950.0119.762661
CTGCGGT14450.0116.760332
GCCCTGT1000.0115.36321
GGACCAT256.647315E-6115.36321
CATGCGG2100.0113.303151
ACTGCGG6500.0113.1446841
Produced by FastQC (version 0.11.5)