FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_2_S10_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_2_S10_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18388474
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG348100.1893033647055215No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCTC100.0072322274144.05251
TGCAGCT100.0072322274144.05251
CCTGGCT1300.0138.512021
CATGGCT550.0130.956821
GCCTGCT1050.0123.4735641
CCTGCGG2450.0120.5337141
GCCATGG256.682154E-6115.2421
ACTGCGG2450.0108.774331
GTGCCCA203.9693143E-4108.0393751
TGTGCTC6400.0106.913962
CTGTGCT2000.0104.438061
CCAGGCT700.0102.894641
CTGGCTC5050.0102.6908952
TTGGCTC6950.0102.598542
GGCCTTT502.055458E-10100.836751
CTGCGGT5400.098.702632
TGGCTCT18800.097.273433
CAGGCTC1800.096.0352
ACTGGCT1500.096.0349961
TGCAGAC4850.095.007223
Produced by FastQC (version 0.11.5)