FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_2_S10_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_2_S10_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18388474
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATGG253.839523E-8144.194981
CCTGCGG4800.0135.182821
CATGGCT1550.0134.892091
CCTGGCT2900.0134.250521
CTTGGCT2650.0133.312351
GGCCTAC450.0128.173321
GCCCTGT700.0123.5956951
CCTGCGA2500.0118.23991
CCAGGCT2050.0112.5424351
GGCCTTA458.913048E-11112.1516651
CTGTGCT1100.0111.42341
TTGGCTC12750.0110.832242
CTGCGGT9200.0109.7135852
GTGCAAC203.9537344E-4108.146251
GCTGCGG4200.0108.146241
CAGGCTC4600.0106.578912
GCCAGCT1100.0104.869081
GGCCATA1450.0104.417071
GCTGGCT1800.0104.140831
GGCCAAA1600.0103.640151
Produced by FastQC (version 0.11.5)