FastQCFastQC Report
Mon 22 Jul 2019
8C_26psu_2_S10_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_2_S10_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18482577
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG384670.20812573917587357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGCT550.0130.95031
CTTGGCT850.0127.0988161
GCTGGCT750.0124.839281
CATGGCT600.0120.0377661
GCCATGG301.1467819E-7120.0377661
CTGTGCT1750.0119.351841
CCTGCGG2700.0117.370261
GCCTGCT1750.0115.236261
CCTGGCT1000.0115.236261
GCCCTTG458.913048E-11112.0352551
GGCCATA1100.0111.3077551
TTGGCTC6700.0108.571482
CTGGCTC4850.0108.405242
CTAGGCT1000.0108.033991
ACTGGCT1150.0106.4682851
TGTGCTC8000.0103.532582
GACTGCT352.8746217E-7102.889521
TCTGGCT1900.0102.347991
ACTGCGG2150.0100.496741
GCTGCGG2600.099.7236941
Produced by FastQC (version 0.11.5)